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477601
477600
C30101
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730,83 CHF
2.399,91 CHF
3.961,40 CHF
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KurzbeschreibungN-CHO CapillaryCarbohydrate Labeling & Analysis KitBioPhase Capillary Isoelectric Focusing (cIEF) kit
Ausführliche BeschreibungNCHO capillary is a coated capillary that contains an inner diameter of 50 µm and is 65 cm in length (Qty: 1). This coated capillary is used for the separation of oligosaccharides and monosaccharides released from glycoproteins utilizing the Carbohydrate Labeling & Analysis Kit (P/N 477600). This capillary can also be used for certain cIEF applications including AAV Full vs. Empty analysis.The Carbohydrate Labeling and Analysis Kit is designed for use on the PA 800 Plus and contains the reagents, buffers, and capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence (LIF) detection. This kit provides reagent volumes for analysis of 100 samples and includes: • Carbohydrate Separation Buffer, 56 mL • N-CHO Coated Capillary, 2 units • Labeling Reagents, 1 vial • Labeling Dye (APTS), 4 x 5 mg • Labeling Dye Solvent, 1 mL • Glucose Ladder Standard, 50 mg • Quantitation/Mobility Marker (Maltose), 0.18 mg • APTS-M (monosaccharide-grade), 20 mgCapillary Isoelectric Focusing (CIEF) assay configured for use on the BioPhase 8800 system. Reagent volumes enable 200 tests. Kit includes: Cathodic Stabilizer Anodic Stabilizer cIEF Gel Urea Neutral Capillary Conditioning Solution Anolyte Catholyte Chemical Mobilizer Formamide CE Grade Water cIEF Protein Analysis Kit
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